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Stable Isotope Labeled Peptides

A stable isotope-labeled peptide is a chemically synthesized peptide with the native sequence, but some of the constituent amino acids are replaced by the stable isotope-labeled amino acids incorporating ²H, ¹³C, and/or ¹⁵N atom(s). Such labeling results in altering the mass of a peptide by as little as a few Da, to as much as tens of Da from that of the parental peptide. LC-MS analysis of the labeled peptide makes the direct and highly efficient quantification of the natural peptide in samples prepared from the living body possible.

Characteristic Features

• Design of your peptide with the expected mass difference by choosing the plural stable isotope-labeled amino acids
• Easy and efficient quantification of the natural peptide by mass spectrometric analysis
• Use as an ordinary way because stable isotope is not radioactive
• Possible assorted modifications such as disulfide bond formation, glycosylation, phosphorylation, etc.
• Direct analysis of samples from the living body without any prior treatments such as reduction/alkylation of disulfide bond(s), enzymatic digestion, etc.
• Applicability to quantification of proteins in the similar way to that using the AQUA peptide

Examples

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