Stable Isotope Labeled Peptides
A stable isotope-labeled peptide is a chemically synthesized peptide with the native sequence, but some of the constituent amino acids are replaced by the stable isotope-labeled amino acids incorporating 2H, 13C, and/or 15N atom(s). Such labeling results in altering the mass of a peptide by as little as a few Da, to as much as tens of Da from that of the parental peptide. LC-MS analysis of the labeled peptide makes the direct and highly efficient quantification of the natural peptide in samples prepared from the living body possible.
- Design of your peptide with the expected mass difference by choosing the plural stable isotope-labeled amino acids
- Easy and efficient quantification of the natural peptide by mass spectrometric analysis
- Use as an ordinary way because stable isotope is not radioactive
- Possible assorted modifications such as disulfide bond formation, glycosylation, phosphorylation, etc.
- Direct analysis of samples from the living body without any prior treatments such as reduction/alkylation of disulfide bond(s), enzymatic digestion, etc.
- Applicability to quantification of proteins in the similar way to that using the AQUA peptide
Code 3404-v 4-[D10]Leu-Insulin (Human) (+40 Da)
Code 3405-v [13C18,15N3]-Hepcidin (Human) (+21 Da)