New Tool for Alzheimer’s Disease Research!
Through functional expression screening (termed a death-trap screening), Professor Nishimoto (KEIO University) and his colleagues identified a cDNA, encoding a novel 24-amino acid-residue peptide, designated as “humanin” [Proc. Natl. Acad. Sci. U.S.A., 98, 6336-6341 (2001)]. This peptide abolishes death of neuronal cells caused by multiple different types of familial Alzheimer’s disease gene products and Aβ amyloid.
Transfection of a plasmid encoding humanin cDNA to F11 neuronal cells suppressed toxicity by either V642I-APP, M146L-PS1, or N141I-PS2 cDNA. The cultured medium of F11 cells transfected with plasmid humanin carried protective activity, and contained bands of the humanin immunoreactivity at 3-4 kDa. Humanin was thus transcribed from coding death-trap clones and secreted into the medium.
Synthetic humanin protected V642I-APP-transfected F11 cells from death in a dose-dependent manner. After structure-activity relationship study, they found potent analog, that is, a Ser to Gly substituted peptide at position 14. This analog is 1000 times more potent than that of the parent peptide humanin. The [Gly14]-humanin(10 nM) blocks V642I-APP, M146L-PS1, and N141I-PS2 cDNA-induced cell-death. When neurons are pretreated with 10 nM of [Gly14]-humanin, Aβ1-43-induced death as well as dystrophic changes of neurites are suppressed.
Although further experiments are required, this potent [Gly14]-humanin will contribute to generate the new Alzheimer’s disease therapeutics targeting for neuroprotection.
|4385-v||[Gly14]-Humanin||0.5 mg vial||¥10,000|
|4384-v||Humanin||0.5 mg vial||¥25,000|
|4379-v||Amyloid β- Protein (Human, 1- 40) [HCl Form]||0.5 mg vial||¥20,000|
|4307-v||Amyloid β- Protein (Human, 1- 40)||0.5 mg vial||¥18,000|
|4349-v||Amyloid β- Protein (Human, 1- 42)||0.5 mg vial||¥30,000|
|4370-v||Amyloid β- Protein (Human, 1- 43)||0.5 mg vial||¥35,000|
|4367-v||[Pyr3]-Amyloid β- Protein (Human, 3- 42)||0.5 mg vial||¥30,000|
|4309-v||Amyloid β- Protein (Human, 25-35)||0.5 mg vial||¥4,000|
|4359-v||Amyloid β- Protein (Human, 1- 16)||0.5 mg vial||¥10,000|
|4358-v||β-Sheet Breaker Peptide iAβ5||5 mg vial||¥16,000|
|14307-v||Amyloid β- Protein (Human, 1- 40) Antiserum||0.05 ml vial||¥25,000|
|14356-v||Amyloid β- Protein (Human, 34- 40) Antiserum||0.05 ml vial||¥25,000|
|14357-v||Amyloid β- Protein (Human, 37- 42) Antiserum||0.05 ml vial||¥25,000|
|14359-v||Amyloid β- Protein (Human, 1- 16) Antiserum||0.05 ml vial||¥25,000|