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Storage:

-20 ℃

Source:

Chinese Hamster Ovarian Cells (CHO).

Production:

LBP is produced from mouse LBP transfected CHO-cells in serum free medium. For transfection we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before transfection the complete mouse LBP cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. RmLBP was produced by his-tag by means of metal affinity purification with Talon and controlled by SDS page. Showing a 58kDa band on SDS-PAGE. Attention: His-tag at the c-terminal end of the LBP has no protease site and is not to split off.