{"id":508,"date":"2013-07-22T18:56:18","date_gmt":"2013-07-22T09:56:18","guid":{"rendered":"http:\/\/120.29.164.89\/?p=508"},"modified":"2023-08-29T10:20:20","modified_gmt":"2023-08-29T01:20:20","slug":"508","status":"publish","type":"post","link":"https:\/\/www.peptide.co.jp\/en\/new-product\/508.html","title":{"rendered":"ProTx-I"},"content":{"rendered":"

T-type Ca2+<\/sup>-Channel Blocker with Na+<\/sup>\/K+<\/sup>-Channel Blocking Activity<\/b><\/p>\n

ProTx-I was isolated from the venom of the tarantula Thrixopelma pruriens<\/i> and its primary structure was determined to consist of 35 amino acid residues with three intrachain disulfide bonds[Biochemistry<\/i> 41<\/b>, 14734 (2002)]. This peptide shows sequence similarity to hanatoxin1 and belongs to a member of the “inhibitor cystine knot” peptide family.<\/p>\n

ProTx-I :<\/b><\/p>\n

    Glu-Cys-Arg-Tyr-Trp-Leu-Gly-Gly-Cys-Ser-\r\n    Ala-Gly-Gln-Thr-Cys-Cys-Lys-His-Leu-Val-\r\n    Cys-Ser-Arg-Arg-His-Gly-Trp-Cys-Val-Trp-\r\n    Asp-Gly-Thr-Phe-Ser<\/pre>\n
Like other venomous toxins, ProTx-I exerts inhibitory activities for each ion channels; i)<\/b> T-type Ca2+<\/sup> channel [Cav<\/sub>3.1 (\u03b11G<\/sub>), IC50<\/sub> = 53 nM], ii)<\/b> Na+<\/sup> channel [Nav<\/sub>1.2, Nav<\/sub>1.5, Nav<\/sub>1.7 (IC50<\/sub> = 51 nM), and Nav<\/sub>1.8 (IC50<\/sub> = 27 nM)], and iii)<\/b> K+<\/sup> channel [Kv<\/sub>2.1 (IC50<\/sub> = 411 nM) and Kv<\/sub>1.3 (40% blocking at 730 nM)].<\/p>\n
ProTx-I thus elicits multiple channel blocking activities with certain selectivity since K+<\/sup> channel inhibitory activity is relatively weak. ProTx-I is one of the first high-affinity ligands to be identified that possess tetrodotoxin-resistant Na+<\/sup> and T-type Ca2+<\/sup> channel blocking activity.  This peptide should prove useful for elucidating the gating mechanism of voltage-dependent ion channels.<\/p>\n\n\n\n\n
Code<\/td>\nCompound<\/td>\nActivity<\/td>\nPackage<\/td>\n<\/tr>\n
 4409-s<\/a><\/td>\nProTx-I<\/td>\nT-type Ca2+<\/sup>\/Na+<\/sup>\/K+<\/sup> Channel Blocker<\/td>\n0.1 mg vial<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
Related Peptide<\/p>\n\n\n\n\n\n\n
Code<\/td>\nCompound<\/td>\nActivity<\/td>\nPackage<\/td>\n<\/tr>\n
4375-s<\/a><\/td>\nKurtoxin<\/td>\nT-type Ca2+<\/sup> Channel Blocker<\/td>\n0.1 mg vial<\/td>\n<\/tr>\n
4217-v<\/a><\/td>\n\u03bc-Conotoxin GIIIB<\/td>\nNa+<\/sup> Channel Blocker<\/td>\n0.5 mg vial<\/td>\n<\/tr>\n
4263-v<\/a><\/td>\n\u03bc-Conotoxin GS<\/td>\nNa+<\/sup> Channel Blocker<\/td>\n0.5 mg vial<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"
T-type Ca2+-Channel Blocker with Na+\/K+-Channel Blocking Activity<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[2],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/posts\/508"}],"collection":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/comments?post=508"}],"version-history":[{"count":1,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/posts\/508\/revisions"}],"predecessor-version":[{"id":4934,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/posts\/508\/revisions\/4934"}],"wp:attachment":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/media?parent=508"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/categories?post=508"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/tags?post=508"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}