{"id":471,"date":"2013-07-22T18:31:05","date_gmt":"2013-07-22T09:31:05","guid":{"rendered":"http:\/\/120.29.164.89\/?p=471"},"modified":"2023-08-29T10:23:53","modified_gmt":"2023-08-29T01:23:53","slug":"471","status":"publish","type":"post","link":"https:\/\/www.peptide.co.jp\/en\/new-product\/471.html","title":{"rendered":"LEAP-2 (Human)"},"content":{"rendered":"<p><b>Antimicrobial Peptide in Human Blood Ultrafiltrate<\/b><\/p>\n<p>One methods aimed at finding peptides and proteins, is the systematic analysis of polypeptides in human blood ultrafiltrate, which has proven to be successful.  Liver-expressed antimicrobial peptide 1 (LEAP-1)\/hepcidin (<a href=\"\/en\/catalog\/f-cat?k_code=4392-s\">Code 4392-s<\/a>) is an example of such, which elicits antimicrobial activity as well as some functions in iron homeostasis.  Following LEAP-1, a new cationic peptide termed LEAP-2 has recently discovered by using this same method, the &#8220;peptide trapping&#8221; approach [<i>Protein Sci.,<\/i> <b>12<\/b>, 143 (2003)].<\/p>\n<p>The isolated peptides have variable chain length with the deletion of the longest peptide, composed of 40 amino acid residues, at both the amino- and carboxyl-termini.  Its disulfide arrangement was determined to be Cys<sup>1<\/sup>-Cys<sup>3<\/sup> and Cys<sup>2<\/sup>-Cys<sup>4<\/sup> (Cys numbering from the amino-terminus) by analyzing the molecular weight and sequence of the tryptic fragments.  The cDNA cloning of LEAP-2 reveals the precursor of LEAP-2 to be a 77-residue peptide, in which the longest form corresponds to the residues between the positions of 38 and 77 in the precursor [prepro-LEAP-2(38-77)].  The primary structures of LEAP-2 of rhesus monkey, cow, pig, mouse, and guinea pig were also determined, and found to be highly conserved among these species. LEAP-2 mRNA is predominantly expressed in liver and small intestine.<\/p>\n<p><b>Liver-Expressed Antimicrobial Peptide 2 (Human)<\/b><\/p>\n<pre>    Met-Thr-Pro-Phe-Trp-Arg-Gly-Val-Ser-Leu-\r\n    Arg-Pro-Ile-Gly-Ala-Ser-Cys-Arg-Asp-Asp-\r\n    Ser-Glu-Cys-Ile-Thr-Arg-Leu-Cys-Arg-Lys-\r\n    Arg-Arg-Cys-Ser-Leu-Ser-Val-Ala-Gln-Glu\r\n\r\n(Disulfide bonds between Cys<sup>17<\/sup>-Cys<sup>28<\/sup> and Cys<sup>23<\/sup>-Cys<sup>33<\/sup>)<\/pre>\n<p>LEAP-2 [preproLEAP-2 (38-77)] exerts antibacterial activities against Gram-positive, Gram-negative bacteria, and yeast (IC<sub>50<\/sub> value was around 5 \u03bcM against <i>Saccharomyces. cerevisiae<\/i>). Interestingly, the amino-terminally truncated form, prepro-LEAP-2 (44-77), has not affected the viability of the germs tested.  This 40 amino acid residue peptide, LEAP-2, has been reported to be circulating in human blood.  LEAP-2 should prove to be varuable in studying its role in self-defense in humans, as well as its role as an antibacterial peptide in the family of the well-established \u03b1-\/\u03b2-defensins.<\/p>\n<table style=\"width: 90%\" border=\"1\">\n<tbody>\n<tr>\n<td align=\"center\">Code<\/td>\n<td align=\"center\">Compound<\/td>\n<td align=\"center\">Package<\/td>\n<\/tr>\n<tr>\n<td><a href=\"\/en\/catalog\/f-cat?k_code=4405-s\">4405-s<\/a><\/td>\n<td>Liver-Expressed Antimicrobial Peptide 2 (Human)<\/td>\n<td align=\"center\">0.1 mg vial<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Related Products<\/p>\n<table style=\"width: 90%\" border=\"1\">\n<tbody>\n<tr align=\"center\">\n<td>Code<\/td>\n<td>Compound<\/td>\n<td>Package<\/td>\n<\/tr>\n<tr>\n<td><a href=\"\/en\/catalog\/f-cat?k_code=4392-s\">4392-s<\/a><\/td>\n<td>Liver-Expressed Antimicrobial Peptide 1 (Human)<\/td>\n<td align=\"center\">0.1 mg vial<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>Novel Inducible Antimicrobial Peptides identified in Human Blood Ultrafiltrate<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[2],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/posts\/471"}],"collection":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/comments?post=471"}],"version-history":[{"count":1,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/posts\/471\/revisions"}],"predecessor-version":[{"id":4941,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/posts\/471\/revisions\/4941"}],"wp:attachment":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/media?parent=471"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/categories?post=471"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/tags?post=471"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}