{"id":3491,"date":"2016-04-13T09:52:32","date_gmt":"2016-04-13T00:52:32","guid":{"rendered":"https:\/\/www.peptide.co.jp\/en\/?p=3491"},"modified":"2023-08-29T10:17:39","modified_gmt":"2023-08-29T01:17:39","slug":"3108","status":"publish","type":"post","link":"https:\/\/www.peptide.co.jp\/en\/new-product\/3108.html","title":{"rendered":"Phopsho Ubiquitin: [Ser(PO3<\/sub>H2<\/sub>)65<\/sup>]-Ubiquitin"},"content":{"rendered":"

In 2014, Koyano et al<\/i>.1)<\/sup> and other groups2)-4)<\/sup> reported a novel function of ubiquitin, that [Ser(PO3<\/sub>H2<\/sub>)65<\/sup>]-ubiquitin (phosphorylated ubiquitin at Ser65<\/sup>) is clarified to be an essential player in mitochondrial quality control. PINK1 (PTEN-induced protein kinase 1) phosphorylates ubiquitin at Ser65<\/sup>, as well as Parkin (E3 ubiquitin ligase) at the similar Ser residue in its ubiquitin-like domain upon depolarization of mitochondrial membrane (mitochondria damage), which follows the activation of latent Parkin E3 by retrieving the catalytic cysteine from autoinhibition.<\/p>\n

\"phosphoUb_en\"<\/p>\n

After this initial observation, research in relation to phosphorylation of ubiquitin has been increasing tremendously, including: i) activation mechanism of Parkin by [Ser(PO3<\/sub>H2<\/sub>)65<\/sup>]-ubiquitin by crystallographic analysis using Pediculus humanus Parkin5)<\/sup>, ii) disposal of damaged mitochondria through mitophagic pathway where [Ser(PO3<\/sub>H2<\/sub>)65<\/sup>]-ubiquitin, phosphorylated by PINK1, is involved in inducing autophagy receptor recruitment6)<\/sup><\/p>\n

These experimental results imply that [Ser(PO3<\/sub>H2<\/sub>)65<\/sup>]-ubiquitin may shed light on the research of Parkinson\u2019s disease which is associated with mitochondrial dysfunction.<\/p>\n

\u3000<\/p>\n\n\n\n\n\n
Code<\/td>\nCompound<\/td>\nQuantity<\/td>\n<\/tr>\n
4486-v <\/a><\/td>\n[Ser(PO3<\/sub>H2<\/sub>)65<\/sup>]-Ubiquitin<\/td>\n50 \u03bcg vial<\/td>\n<\/tr>\n
3207-v <\/a><\/td>\nUbiquitin Aldehyde<\/td>\n50 \u03bcg vial<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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Feel free to contact us if you have any questions.<\/a><\/Div><\/p>\n
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  1. Nature<\/i>, 510<\/B>, 162 (2014). DOI: 10.1038\/nature13392<\/a> (Original)<\/i><\/li>\n
  2. J. Cell Biol.<\/i>, 205<\/B>, 143 (2014). DOI: 10.1083\/jcb.201402104<\/a> (Original)<\/i><\/li>\n
  3. Biochem. J.<\/i>, 460<\/B>, 127 (2014). DOI: 10.1042\/BJ20140334<\/a> (Original)<\/i><\/li>\n
  4. Mol. Cell<\/i>, 56<\/B>, 360 (2014). DOI: 10.1016\/j.molcel.2014.09.007<\/a> (Original)<\/i><\/li>\n
  5. Nature<\/i>, 524<\/B>, 370 (2015). DOI: 10.1038\/nature14879<\/a> (Role in Parkin Activation)<\/i><\/li>\n
  6. Nature<\/i>, 524<\/B>, 309 (2015). DOI: 10.1038\/nature14893<\/a> (Role in Mitophagy)<\/i><\/li>\n

    \u3000
    \n\u3000<\/p>\n","protected":false},"excerpt":{"rendered":"

    In 2014, Koyano et al.1) and other groups2)-4) reported a novel function of ubiquitin, that [Ser(PO3H2)65]-ubi […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[2],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/posts\/3491"}],"collection":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/comments?post=3491"}],"version-history":[{"count":6,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/posts\/3491\/revisions"}],"predecessor-version":[{"id":4929,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/posts\/3491\/revisions\/4929"}],"wp:attachment":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/media?parent=3491"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/categories?post=3491"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/tags?post=3491"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}