{"id":308,"date":"2013-07-19T16:15:43","date_gmt":"2013-07-19T07:15:43","guid":{"rendered":"http:\/\/120.29.164.89\/?p=308"},"modified":"2023-08-29T11:08:41","modified_gmt":"2023-08-29T02:08:41","slug":"308","status":"publish","type":"post","link":"https:\/\/www.peptide.co.jp\/en\/new-product\/308.html","title":{"rendered":"Cathepsin D\/E Substrate MOCAc-Gly-Lys-Pro-Ile-Leu-Phe- Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2"},"content":{"rendered":"<p>Intracellular proteases are focused for studying a control system of the cellular function. Many proteases, like cathepsins, calpains, caspases, and enzymes for ubiquitin-proteasome system, are controlling the cellular functions with interaction each other.<\/p>\n<p><img loading=\"lazy\" src=\"\/wp-content\/uploads\/2013\/07\/cathepsinde.gif\" alt=\"cathepsinde\" width=\"398\" height=\"168\" class=\"alignnone size-full wp-image-2190\" \/><\/p>\n<p>Cathepsin E and cathepsin D are two major intracellular aspartic proteinases implicated in the physiological and pathological degradation of intra- and extracellular proteins. Thus far, there are no useful synthetic substrates provide with a simple and fast method for measuring enzyme activities of large numbers of samples.<\/p>\n<p>Recently, Yasuda et al. reported new fluorogenic substrate for the rapid and sensitive assay of cathepsin E and cathepsin D [<i>J. Biochem.<\/i> <b>125<\/b>, 1137 (1999)]. This substrate is hydrolyzed equally well by cathepsins E and D (kcat\/Km = 10.9 mM<sup>-1<\/sup> \u00b7 s<sup>-1<\/sup> for cathepsin E and 15.6 mM<sup>-1<\/sup> \u00b7 s<sup>-1<\/sup> for cathepsin D). This substrate represents a potentially valuable tool for mechanistic studies on cathepsins E and D.<\/p>\n<table style=\"width: 90%\" border=\"1\">\n<tbody>\n<tr align=\"center\">\n<td>Code<\/td>\n<td>Compound<\/td>\n<td>Package<\/td>\n<\/tr>\n<tr>\n<td><a href=\"\/en\/catalog\/f-cat?k_code=3200-v\">3200-v<\/a><\/td>\n<td>MOCAc-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH<sub>2<\/sub><\/td>\n<td align=\"center\">1 mg vial<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"<p>Intracellular proteases are focused for studying a control system of the cellular function. Many proteases, li [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[2],"tags":[],"acf":[],"_links":{"self":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/posts\/308"}],"collection":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/comments?post=308"}],"version-history":[{"count":1,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/posts\/308\/revisions"}],"predecessor-version":[{"id":4961,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/posts\/308\/revisions\/4961"}],"wp:attachment":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/media?parent=308"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/categories?post=308"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/tags?post=308"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}