{"id":72,"date":"2013-07-09T14:23:32","date_gmt":"2013-07-09T05:23:32","guid":{"rendered":"http:\/\/120.29.164.89\/?page_id=72"},"modified":"2013-07-09T14:23:32","modified_gmt":"2013-07-09T05:23:32","slug":"labeled-peptides","status":"publish","type":"page","link":"https:\/\/www.peptide.co.jp\/en\/custom\/peptide_synthesis\/labeled-peptides","title":{"rendered":"Stable Isotope Labeled Peptides"},"content":{"rendered":"<p>A stable isotope-labeled peptide is a chemically synthesized peptide with the native sequence, but some of the constituent amino acids are replaced by the stable isotope-labeled amino acids incorporating <sup>2<\/sup>H, <sup>13<\/sup>C, and\/or <sup>15<\/sup>N atom(s). Such labeling results in altering the mass of a peptide by as little as a few Da, to as much as tens of Da from that of the parental peptide. LC-MS analysis of the labeled peptide makes the direct and highly efficient quantification of the natural peptide in samples prepared from the living body possible.<\/p>\n<h3>Characteristic Features<\/h3>\n<ul>\n<li>Design of your peptide with the expected mass difference by choosing the plural stable isotope-labeled amino acids<\/li>\n<li>Easy and efficient quantification of the natural peptide by mass spectrometric analysis<\/li>\n<li>Use as an ordinary way because stable isotope is not radioactive<\/li>\n<li>Possible assorted modifications such as disulfide bond formation, glycosylation, phosphorylation, etc.<\/li>\n<li>Direct analysis of samples from the living body without any prior treatments such as reduction\/alkylation of disulfide bond(s), enzymatic digestion, etc.<\/li>\n<li>Applicability to quantification of proteins in the similar way to that using the AQUA peptide<\/li>\n<\/ul>\n<h3>Examples<\/h3>\n<div class=\"floatbox span1-1\">\n<p>Code <a href=\"https:\/\/www.peptide.co.jp\/en\/catalog\/f-cat?k_code=3404-v\">3404-v<\/a> 4-[D10]Leu-Insulin (Human) <span style=\"color: #ff0000\">(+40 Da)<\/span><\/p>\n<p class=\"aligncenter\" style=\"margin-top: 1em\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1362\" alt=\"3404-v\" src=\"https:\/\/www.peptide.co.jp\/en\/wp-content\/uploads\/2013\/08\/206-1.gif\" width=\"541\" height=\"102\" \/><\/p>\n<\/div>\n<div class=\"floatbox span1-1\">\n<p>Code <a href=\"https:\/\/www.peptide.co.jp\/en\/catalog\/f-cat?k_code=3405-v\">3405-v<\/a>\u3000[<sup>13<\/sup>C<sub>18<\/sub>,<sup>15<\/sup>N<sub>3<\/sub>]-Hepcidin (Human) <span style=\"color: #ff0000\">(+21 Da)<\/span><\/p>\n<p class=\"aligncenter\" style=\"margin-top: 1em\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-1361\" alt=\"3405-v\" src=\"https:\/\/www.peptide.co.jp\/en\/wp-content\/uploads\/2013\/08\/206-2.gif\" width=\"463\" height=\"78\" \/><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>A stable isotope-labeled peptide is a chemically synthesized peptide with the native sequence, but some of the constituent amino acids are replaced by the stable isotope-labeled amino acids incorporating 2H, 13C, and\/or 15N atom(s). Such labeling results in altering the mass of a peptide by as little as a few Da, to as much as tens of Da from that of the parental peptide. LC-MS analysis of the labeled peptide makes the direct and highly efficient quantification of the natural peptide in samples prepared from the living body possible. Characteristic Features Design of your peptide with the expected mass difference by choosing the plural stable isotope-labeled amino acids Easy and [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":999,"parent":65,"menu_order":3406,"comment_status":"open","ping_status":"closed","template":"page-custom.php","meta":{"footnotes":""},"class_list":["post-72","page","type-page","status-publish","has-post-thumbnail","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/pages\/72","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/comments?post=72"}],"version-history":[{"count":0,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/pages\/72\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/pages\/65"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/media\/999"}],"wp:attachment":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/media?parent=72"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}