{"id":72,"date":"2013-07-09T14:23:32","date_gmt":"2013-07-09T05:23:32","guid":{"rendered":"http:\/\/120.29.164.89\/?page_id=72"},"modified":"2013-07-09T14:23:32","modified_gmt":"2013-07-09T05:23:32","slug":"labeled-peptides","status":"publish","type":"page","link":"https:\/\/www.peptide.co.jp\/en\/custom\/peptide_synthesis\/labeled-peptides","title":{"rendered":"Stable Isotope Labeled Peptides"},"content":{"rendered":"<p>A stable isotope-labeled peptide is a chemically synthesized peptide with the native sequence, but some of the constituent amino acids are replaced by the stable isotope-labeled amino acids incorporating <sup>2<\/sup>H, <sup>13<\/sup>C, and\/or <sup>15<\/sup>N atom(s). Such labeling results in altering the mass of a peptide by as little as a few Da, to as much as tens of Da from that of the parental peptide. LC-MS analysis of the labeled peptide makes the direct and highly efficient quantification of the natural peptide in samples prepared from the living body possible.<\/p>\n<h3>Characteristic Features<\/h3>\n<ul>\n<li>Design of your peptide with the expected mass difference by choosing the plural stable isotope-labeled amino acids<\/li>\n<li>Easy and efficient quantification of the natural peptide by mass spectrometric analysis<\/li>\n<li>Use as an ordinary way because stable isotope is not radioactive<\/li>\n<li>Possible assorted modifications such as disulfide bond formation, glycosylation, phosphorylation, etc.<\/li>\n<li>Direct analysis of samples from the living body without any prior treatments such as reduction\/alkylation of disulfide bond(s), enzymatic digestion, etc.<\/li>\n<li>Applicability to quantification of proteins in the similar way to that using the AQUA peptide<\/li>\n<\/ul>\n<h3>Examples<\/h3>\n<div class=\"floatbox span1-1\">\n<p>Code <a href=\"https:\/\/www.peptide.co.jp\/en\/catalog\/f-cat?k_code=3404-v\">3404-v<\/a> 4-[D10]Leu-Insulin (Human) <span style=\"color: #ff0000\">(+40 Da)<\/span><\/p>\n<p class=\"aligncenter\" style=\"margin-top: 1em\"><img loading=\"lazy\" class=\"alignnone size-full wp-image-1362\" alt=\"3404-v\" src=\"https:\/\/www.peptide.co.jp\/en\/wp-content\/uploads\/2013\/08\/206-1.gif\" width=\"541\" height=\"102\" \/><\/p>\n<\/div>\n<div class=\"floatbox span1-1\">\n<p>Code <a href=\"https:\/\/www.peptide.co.jp\/en\/catalog\/f-cat?k_code=3405-v\">3405-v<\/a>\u3000[<sup>13<\/sup>C<sub>18<\/sub>,<sup>15<\/sup>N<sub>3<\/sub>]-Hepcidin (Human) <span style=\"color: #ff0000\">(+21 Da)<\/span><\/p>\n<p class=\"aligncenter\" style=\"margin-top: 1em\"><img loading=\"lazy\" class=\"alignnone size-full wp-image-1361\" alt=\"3405-v\" src=\"https:\/\/www.peptide.co.jp\/en\/wp-content\/uploads\/2013\/08\/206-2.gif\" width=\"463\" height=\"78\" \/><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>A stable isotope-labeled peptide is a chemically synthesized peptide with the native sequence, but some of the [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":999,"parent":65,"menu_order":3406,"comment_status":"open","ping_status":"closed","template":"page-custom.php","meta":[],"acf":[],"_links":{"self":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/pages\/72"}],"collection":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/comments?post=72"}],"version-history":[{"count":0,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/pages\/72\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/pages\/65"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/media\/999"}],"wp:attachment":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/media?parent=72"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}