{"id":66,"date":"2013-07-09T14:18:48","date_gmt":"2013-07-09T05:18:48","guid":{"rendered":"http:\/\/120.29.164.89\/?page_id=66"},"modified":"2023-08-21T09:36:12","modified_gmt":"2023-08-21T00:36:12","slug":"long_peptides","status":"publish","type":"page","link":"https:\/\/www.peptide.co.jp\/en\/custom\/peptide_synthesis\/long_peptides","title":{"rendered":"Long Peptides and Proteins"},"content":{"rendered":"<p class=\"catchphrase\">Do not hesitate to contact us even when your peptide consists of over 100 residues.<\/p>\n<p>We have successfully synthesized green fluorescent protein (GFP) with 238 amino acids. <span class=\"ref\">[Proc. Natl. Acad. Sci. U.S.A., <b>95<\/b>, 13549 (1998).]<\/span><\/p>\n<ul>\n<li>Chemical synthesis of the long peptides and proteins with high purity by solution\/solid phase synthesis, Boc\/Bzl or Fmoc\/<em>t<\/em>Bu chemistry.<\/li>\n<li>Advantage: site specific modification of your molecule. (fluorescence-labeling, stable isotope-labeling, glycosylation, phosphorylation, etc.)<\/li>\n<\/ul>\n<h3>Our Results<\/h3>\n<ul>\n<li>GFP <b>238AA<\/b><\/li>\n<li>Code <a href=\"https:\/\/www.peptide.co.jp\/en\/catalog\/f-cat?k_code=4298-v\">4298-v<\/a> Midkine (Human) <b>121AA<\/b> 5SS<\/li>\n<li>Code <a href=\"https:\/\/www.peptide.co.jp\/en\/catalog\/f-cat?k_code=3224-s\">3224-s<\/a> FRETS-VWF73 <b>73AA<\/b><br \/>\n<img loading=\"lazy\" class=\"alignnone\" style=\"margin-top: 5px;\" src=\"\/wp-content\/uploads\/2013\/08\/201-1_en1.gif\" alt=\"3224-s\" width=\"564\" height=\"187\" \/><\/li>\n<\/ul>\n<h4>Purity of FRETS-VWF73 from other supplier<\/h4>\n<p><img loading=\"lazy\" class=\"alignleft\" src=\"\/wp-content\/uploads\/2013\/08\/201-2_en.gif\" alt=\"FRETS-VWF73\" width=\"276\" height=\"251\" \/><\/p>\n<p class=\"catchphrase alignleft span1-2\" style=\"text-align: left;\">Do you think broadening of the peak on RP-HPLC analysis being the intrinsic property of the long peptide and protein? Peptide Institute, Inc. guarantees the purity of our synthetic long peptides and proteins to be high enough by applying several highly efficient analytical methods.<\/p>\n<div class=\"memo alignleft\"><i class=\"fa fa-square\"><\/i> Analytical methods<br \/>\n<span class=\"memo_h\">Column:<\/span>YMC Pack ODS-A (4.6 mm I. D. x 150 mm)<br \/>\n<span class=\"memo_h\">Eluent:<\/span>0.1 M NaCl (pH 2.4)<br \/>\n<span class=\"memo_h\">Gradient:<\/span>Acetonitrile 20% to 60% [25 min]<br \/>\n<span class=\"memo_h\">Flow Rate:<\/span>1.0 mL\/min<br \/>\n<span class=\"memo_h\">Detection:<\/span>210 nm<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Do not hesitate to contact us even when your peptide consists of over 100 residues. We have successfully synth [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":999,"parent":65,"menu_order":3401,"comment_status":"open","ping_status":"open","template":"page-custom.php","meta":[],"acf":[],"_links":{"self":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/pages\/66"}],"collection":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/comments?post=66"}],"version-history":[{"count":4,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/pages\/66\/revisions"}],"predecessor-version":[{"id":4913,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/pages\/66\/revisions\/4913"}],"up":[{"embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/pages\/65"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/media\/999"}],"wp:attachment":[{"href":"https:\/\/www.peptide.co.jp\/en\/wp-json\/wp\/v2\/media?parent=66"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}